How to Use BIO RAD QUANTITY ONE V22 LND zip Software for 1-D Gel Analysis
BIO RAD QUANTITY ONE V22 LND zip is a software package that allows you to acquire, quantitate, and analyze data from 1-D electrophoresis gels, western blots, dot blots, slot blots, arrays, and colony counts. It is compatible with Windows and Macintosh operating systems and supports various Bio-Rad imaging systems, such as densitometers, storage phosphor imagers, fluorescence imagers, and gel documentation systems.
In this article, we will show you how to use BIO RAD QUANTITY ONE V22 LND zip software for 1-D gel analysis, including image acquisition, lane and band detection, molecular weight determination, background subtraction, quantitation, annotation, and spot cutting.
To acquire an image from a Bio-Rad imaging system, you need to connect the device to your computer and launch the BIO RAD QUANTITY ONE V22 LND zip software. Then, select File > New > Acquire and choose the device from the list. The software will automatically configure the device with appropriate filters, lasers, LEDs, and other illumination sources. You can also adjust the settings manually if needed.
Next, place your gel or blot on the device and click Preview to see a low-resolution image of your sample. You can use the tools on the toolbar to zoom in or out, rotate, crop, or adjust the contrast of the image. When you are satisfied with the preview image, click Acquire to capture a high-resolution image of your sample. The image will be displayed in a new window.
Lane and Band Detection
To analyze your 1-D gel image, you need to define the lanes and bands on the gel. To do this automatically, select Analyze > Detect Lanes. The software will scan the image and draw red boxes around each lane. You can use the tools on the toolbar to add or delete lanes, merge or split lanes, or adjust the lane width or position.
Next, select Analyze > Detect Bands. The software will scan each lane and draw blue boxes around each band. You can use the tools on the toolbar to add or delete bands, merge or split bands, or adjust the band width or position.
Molecular Weight Determination
To determine the molecular weight of each band on your gel, you need to have a lane with a molecular weight standard. To do this automatically, select Analyze > MW Determination. The software will ask you to select a lane with a standard and choose a preset standard from the list or enter your own values. Then, it will calculate the molecular weight of each band using one of the regression models available (linear, logarithmic, exponential, etc.). You can also adjust the curve fit manually if needed.
The software will display the molecular weight values on each band and show a graph of the standard curve on the side panel. You can use the tools on the toolbar to change the font size or color of the values, hide or show the graph, or export the data to a spreadsheet.
To correct for uneven background on your gel image, you need to subtract it from each lane. To do this automatically,
select Analyze > Background Subtraction. The software will ask you to choose a method for background subtraction (rolling ball,
it will apply it to each lane and show you a preview of